Karl Haushalter
Assistant Professor of Chemistry and Biology

• Jacobs Science Center, Room 2313
  301 Platt Blvd.
  Claremont, CA 91711
• (909) 607-3928
• Karl_Haushalter@hmc.edu

Education & professional experience

• B.A., Rice University
• Ph.D., Harvard University
• Visiting Lecturer, University of California, Irvine
• Postdoctoral Fellow, University of California, San Diego

Teaching

• Bio 182: Chemistry in Living Systems
• Bio 184: Methods in Biochemistry
• Bio 189: Topics in Biochemistry and Molecular Biology
• Chem 21: General Chemistry
• Chem 25: General Chemistry Laboratory
• Chem 58: Carbon Compounds Laboratory
• Chem 105: Organic Chemistry
• Chem 111: Organic Chemistry Laboratory

Research Interests

Professor Haushalter’s research group uses biochemical and molecular genetic techniques to probe the function of chromatin. They are currently investigating how eukaryotic DNA repair enzymes process lesions embedded in chromatin and the extent to which base-flipping DNA by DNA glycosylases distorts nucleosome structure.

Recent Student Projects

Matthew Hoss (’08) and Sara Hummel (’06): “Sequence Specificity of Human 8-Oxoguanine DNA Glycosylase"

Katie Mouzakis (’07), Yan Pu (’06), and Tiffany Wu (’06): “DNA Repair Efficiency of Variants of Human 8-oxoguanine DNA Glycosylase 1 (hOGG1) Identified in Cancer Patients”

Fang-Yuan Chang (’07): “Trapping the hOGG1:nucleosomal DNA complex”

Brianna Lyon-Robers (’06): “Improved methods for in vitro nucleosome reconstitution”

Steven Petesch (’05): “Location mechanism of adenine·8-oxoguanine mismatches by MutY adenine DNA glycosylase”

Sarah Bundick (’05) and Ryan Mashiyama (’04): “Target Location by the DNA Repair Enzyme SMUG1”

Kit Rodolfa (’04): “Synthesis of large fragments of DNA incorporating site-specific base lesions”

Alexis Kaushansky (’04): “Filling in the black box between I-CreI induced DNA cleavage and double-strand break repair”

Selected Publications

Gemmen, G. J., R. Sim, K. A. Haushalter, P. C. Ke, J. T. Kadonaga, and D. E. Smith. 2005. Forced unraveling of nucleosomes assembled on heterogeneous DNA using core histones, NAP-1, and ACF. J. Mol. Biol. 351: 89-99. [abstract]

Wibley, J. E. A., T. R. Waters, K. Haushalter, G. L. Verdine, and L. H. Pearl. 2003. Structure and specificity of the vertebrate anti-mutator uracil-DNA glycosylase SMUG1. Mol. Cell 11(6): 1647-1659. [article]

K.A. Haushalter, and J. T. Kadonaga. 2003. Chromatin assembly by DNA-translocating motors. Nat. Rev. Mol. Cell Biol. 4(8): 613-620. [abstract]

L. Chen, K. A. Haushalter, C. M. Lieber, and G. L. Verdine. 2002. Direct visualization of a DNA glycosylase searching for damage. Chem. Biol. 9: 345-350. [article]

H. Nilsen, K. A. Haushalter, P. Robins, D. E. Barnes, G. L. Verdine, and T. Lindahl. 2001. Excision of deaminated cytosine from the vertebrate genome: role of the SMUG1 uracil DNA glycosylase. EMBO J. 20: 4278-4286. [abstract]

K. A. Haushalter, P. T. Stukenberg, M. W. Kirschner, and G. L. Verdine. 1999. Identification of a new uracil DNA glycosylase family by expression cloning using synthetic inhibitors. Curr. Biol. 9: 174-185. [article]

K. A. Haushalter, J. Lau, and J. D. Roberts. 1996. An NMR investigation of the effect of hydrogen bonding on the rates of rotation about the C-N bonds in urea and thiourea. J. Amer. Chem. Soc. 118: 8891-8896. [abstract]